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Article Title: The type I activin receptor ActRIB is required for egg cylinder organization and gastrulation in the mouse

doi:

Figure Lengend Snippet: Analysis of expression of lineage-specific genes in the ActRIB−/− embryos. Hemotoxylin and eosin (H/E) stained sections (A,E) and immunostaining with SSEA-1 (B,F), Troma-1 (C,G) and anti-laminin (D,H) antibodies of the wild-type (A–D) and mutant (E–H) embryo sections at E6.5. The section in A is adjacent to that in B; the section in E is adjacent to that in F. Other sections are from different embryos. In each panel, the ectoplacental cone (or the proximal region) is positioned toward the top. The two clusters of stained epiblast cells in E are marked with arrowheads.

Article Snippet: The primary monoclonal or polyclonal antibodies used are Troma-1 (1:100); SSEA-1 (1:100) (from Developmental Studies Hybridoma Bank), and anti-laminin antibody (1: 200) (Sigma).

Techniques: Expressing, Staining, Immunostaining, Mutagenesis

Journal: British Journal of Cancer

Article Title: Tissue biomarkers of breast cancer and their association with conventional pathologic features

doi: 10.1038/bjc.2012.552

Figure Lengend Snippet: Immunological validation of ubiquitin and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.

Article Snippet: To confirm the identity of the m/z 8558 protein peak by protein chip immunocapture, pre-activated RS100 protein chips (Bio-Rad) were pre-coupled with 2 μ g of monoclonal anti-human ubiquitin antibody (R&D) in 50 mℳ NaHCO 3 buffer (pH 9.2) at 4 °C.

Techniques: Western Blot, Mass Spectrometry, Recombinant

Journal: British Journal of Cancer

Article Title: Tissue biomarkers of breast cancer and their association with conventional pathologic features

doi: 10.1038/bjc.2012.552

Figure Lengend Snippet: Association of two protein markers and their combination with tumour histopathologic variables

Article Snippet: To confirm the identity of the m/z 8558 protein peak by protein chip immunocapture, pre-activated RS100 protein chips (Bio-Rad) were pre-coupled with 2 μ g of monoclonal anti-human ubiquitin antibody (R&D) in 50 mℳ NaHCO 3 buffer (pH 9.2) at 4 °C.

Techniques: